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Chromatographic Fingerprint Profile of Methanol Extracts from Non-Conventional Vegetable Cassia tora L. Through HPTLC

Sahadeo Patil and Sushilkumar Wankhade
Department of Microbiology, Shri Shivaji Science College, Amravati - 444603 (MS), India
Abstract—Objective: To set up the chemical profile of methanol extracts of Cassia tora L. by HPTLC technique. Methods: Methanolic extracts prepared from root, stem, leaf, flower and seed of Cassia tora L. using Soxhlet apparatus. HPTLC analysis was done using CAMAG HPTLC system equipped with automatic TLC sampler IV, TLC scanner 3, REPROSTAR 3 with 12 bit CCD camera for photo documentation, winCATS Planer Chromatography software. Results: The results of qualitative phytochemical analysis showed the presence of flavonoids, saponins and tannins in seed extract. Flower and root extract showed the presence of alkaloids, flavonoids, saponins, tannins and terpenoids. Leaf extract results indicate the presence of alkaloids, flavonoids, tannins and terpenoids. However, stem extract confirmed presence of relatively narrow groups of phytochemicals, viz., alkaloids and terpenoids. HPTLC determination of root extract showed the presence of 10 components with Rf values ranging from 0.16 - C 0.85; seed revealed the presence of 9 components with Rf values in the range of 0.09 - C 0.86; leaf extract displayed the presence of 12 components with Rf values in the range of 0.07 - C 0.74; flower (7 components) with Rf values in the range of 0.13 - C 0.86 and stem revealed the presence of 12 components with Rf values ranging from 0.10 - C 0.89. Conclusion: The presence of flavonoids, terpenoids, saponins, tannins and alkaloid content showed that the whole Cassia tora L. can be a natural source of medicine. The HPTLC finger printing of Cassia tora L. may be useful in differentiating the species and act as a biochemical marker for this plant. Further, study will be concentrated on isolation and identification of antibacterial compounds.  

Index Terms—HPTLC, medicinal plant, cassia tora, phytochemicals, biomarkers 

Cite: Sahadeo Patil and Sushilkumar Wankhade, "Chromatographic Fingerprint Profile of Methanol Extracts from Non-Conventional Vegetable Cassia tora L. Through HPTLC," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 2, pp. 146-158, April 2014.
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