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In Vitro Micropropagation of Amomum subulatum (Zingiberaceae), A Major Traditional Cash Crop of Sikkim Himalaya

Sushen Pradhan, Smrita Pradhan, Bharat C Basistha, and K B Subba
Sikkim State Council of Science and Technology, Sajong, Rumtek, East Sikkim
Abstract—Present investigation was achieved with an efficient, simple micropropagation methods was standardized for Ammomum subalatum cv. Ramsey for the production of disease free and rapid multiplication of planting material of Large cardomom micro-propagules and conservation of genetic resources of large cultivator variety of Sikkim. Modified MS medium with 4 % of sucrose with different concentration of hormone showed the suitable medium for multiplication of large cardamom. A total of 52 different hormone concentrations of growth hormone/ cultured medium were examined. The maximum number of shoots and roots were observed on (MS+ sucrose 40 g + BAP 3mg/l+0.5 NAA+2mg/l) and second was (MS+ 40g sucrose + BAP 3.5mg/l+0.5 NAA + 2mg/l). After individually subcultured on same fresh medium, adventitious buds were initiated after 10–15 days of culture, complete plantlets developed within 50 days and maintained for 18 months without decreasing the multiplication rate. The combination of cytokinins and auxins in the culture medium enhanced the response in a number of plants in terms of shoot and root growths, with 100% in vitro multipled plantlets were successfully transferred into field. 

Index Terms—ammomum subalatum, micropropagation, zingibereaceae, ramsey, sikkim

Cite: Sushen Pradhan, Smrita Pradhan, Bharat C Basistha, and K B Subba, "In Vitro Micropropagation of Amomum subulatum (Zingiberaceae), A Major Traditional Cash Crop of Sikkim Himalaya," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 2, pp. 169-180, April 2014.
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