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Isolation and Partial Characterization of Lipase from Bacillus pseudofirmus from Alkaline Lonar Lake

Tambekar D H, Bhosle S V, and Bombode V B
PG Department of Microbiology, SGB Amravati University, Amravati
Abstract—Alkaline Lonar Lake is a unique ecosystem situated in the Buldhana District of the Maharashtra State, India, harbors various haloalkaliphilic bacterial bacillus species which produces biotechnologically important thermo-haloalkaliphilic enzymes. The bacterial strains BS1(1), CS4(1) and DW1(1) were isolated and screened for production and partial characterizations of lipase. The bacterial Isolates were characterized and identified as Bacillus pseudofirmus BS1(1), B. pseudofirmus CS4(1) and B. pseudofirmus DW1(1). Alkaline lipase production was maximum at pH 9 and activity was 1.54 unit/mL to 1.66 unit/mL. Lipase from these bacterial strains was is active at higher temperature and pH and finds potential applications in food, pharmaceutical and detergent industries. 

Index Terms—lonar lake, haloalkaliphiles, bacillus, lipase

Cite: Tambekar D H, Bhosle S V, and Bombode V B, "Isolation and Partial Characterization of Lipase from Bacillus pseudofirmus from Alkaline Lonar Lake," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 3, pp. 85-91, July 2014.
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