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Development of Higher Temperature Tolerant Mutant of Beauveria bassiana and Verticillum lecanii

Avanti1, K Balaraman2, and R Gopinath2
1. Mahtama Gandhi Missions Institute of Biosciences and Technology, Aurangabad, Maharashtra, India 431003
2. Gokulam Biotech, Kannikoil, Pondichery, India 607402
Abstract—The natural populations of insects are attacked by using fungal pathogen of Beauveria bassiana and Verticillium lecanii. Thermotolerant mutants of B. bassiana and V. lecanii were yielded after exposing to the moist heat and UV treatment can offer effective control in all season. The mutants of B. bassiana (BBMh1) and V. lecanii (VLMh1) were yielded maximum biomass and blastospore at 27°C and 35°C as compare to wild type after moist heat treatment. The mutants obtained at 40°C were yields very less biomass and blastospore. Fungal spore suspension were taken for respective treatment at varying time interval of 5, 10, 15, 20 and 30 min. The eight mutants were obtained on the basis of thermotolerant characteristics. 

Index Terms—beauveria bassiana, verticillium lecanii, thermotolerant, biomass, blastospores

Cite: B Avanti, K Balaraman, and R Gopinath, "Development of Higher Temperature Tolerant Mutant of Beauveria bassiana and Verticillum lecanii," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 3, pp. 109-112, July 2014.
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