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Screening and Identification of PUFA Producers from Various Fish of Indian Coastal and Fresh Water Habitats

Azmina A K Masurkar1, Ajit G Datar2, and Babu V Vakil1
1. Guru Nanak Institute for Research and Development, GN Khalsa College, Matunga, Mumbai 400 019
2. Shimadzu Analytical India Pvt. Ltd. Andheri, Mumbai, India 400 059
Abstract—Polyunsaturated Fatty Acids (PUFA) derived from fish exhibit beneficial physiological effects and have well-known nutritional and pharmaceutical significance and are commercially important as additives in food supplements. Microorganisms isolated from fish gut have been reported to be capable of producing PUFA. In our study 49 lipid producing microbes were isolated from intestines of various fresh water and salt water oily fish. The isolates were shortlisted following a preliminary screening scheme by studying a set of sequential parameters like intensity of lipid accumulation as judged by Sudan Black B staining, amount of biomass on nitrogen limited semi-synthetic medium, lipid quantity per gram biomass, relative quantities of saturated and unsaturated fatty acids and reproducibility of results. Ten shortlisted isolates were screened for PUFA production by Gas Chromatography after samples for GC analysis were prepared by extracting the lipids in organic solvent mixture followed by transmethylation with methanolic HCl to form Fatty Acid Methyl Esters (FAMEs). Further, GC/MS analysis confirmed the GC results as five isolates were shown to be capable of producing omega-6(LA) and omega-3(ALA) PUFA. Selected cultures have been identified by 16S r-RNA sequencing. These cultures are being optimized for PUFA production by the aerobic fermentation route. 

Index Terms—polyunsaturated fatty acids (PUFA), fresh water and salt water fish, screening for microbial PUFA producers, GC, GC/MS

Cite: Azmina A K Masurkar, Ajit G Datar, and Babu V Vakil, "Screening and Identification of PUFA Producers from Various Fish of Indian Coastal and Fresh Water Habitats," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 3, pp. 181-195, July 2014.
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