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Choosing of Good White Rot Fungi for Biobleaching of Unbleached Kraft Pulp

D Seshikala
Department of Environmental Science, University Colege of Sciences, Osmania University
Abstract—Considering potentialites of white rot basidiomycetes in biobleaching proces—37 white rot fungi were colected from diferent forest areas of Andhra Pradesh, India. Al of them were screned for lignolytic enzyme production. Out of them 25 diferent organisms were with lignolytic capacity. Then they were quantiatively and qualitatively analyzed for Lacase, LiP and MnP enzymes. Among the studied organisms Stereum ostrea (Lacase 40.02U/L ,MnP 51.59U/L, LiP 1.87U/ L), Tremela frondosa (Lacase 35.07U/L, MnP 29.12U/L, LiP 5.95U/L) Tremates versicolour (MnP and LiP production i.e 56.13 U/L, LiP 23.26 U/L) could show maximum enzyme production. Al the 25 organisms could produce Lacase but few failed to produce MnP and LiP. The organisms which produced both enzymes were grown in the liquid cultures. That culture filtrate was used for qualitative (SDS PAGE). The selected 2 organisms were further studied for biobleaching of unbleached kraft pulp (UKP) where in cu+2 and Mn+2 influence was also studied. 

Index Terms—basidiomycetes, lacase , lignolytic enzymes, MnP , UKP, white rot fungi

Cite: D Seshikala, "Choosing of Good White Rot Fungi for Biobleaching of Unbleached Kraft Pulp," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 3, pp. 208-218, July 2014.
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