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Establishment of In Vitro and Temporarily Immersed Cultures Using Rita of Marchantia Linearis Lehm. and Lindenb. A Liverwort

Remya Krishnan and K Murugan
Plant Biochemistry and Molecular Biology Laboratory, Department of Botany, University College, Thiruvananthapuram, 695 034, Kerala, India
Abstract—The use of in vitro techniques for conservation has been rising steadily since their inclusion in The Convention on Biological Diversity and The Global Strategy for Plant Conservation. Unfortunately, bryophytes are often overlooked in conservation initiatives, but they are important ecologically and medicinally. Axenic culturing of bryophytes seems to be so complicated due to possible interaction with other organisms in non axenic condition. Marchantia linearis Lehm. and Lindenb. a liverwort distributed in restricted scattered localities along the western Ghats and was massively exploited by the Kani tribes for curing many skin disorders. The objective of this study was to develop an efficient in vitro propagation protocol for M. linearis. Multiple leafy thalli were induced significantly on MS/5 medium containing 2 mg/L BA + 0.5 mg/L KIN +0.2 mg/ L Naphthalene Acetic Acid (NAA) than other hormonal combinations. Rhizoid formation was significant with 0.2 mg/L indole-3-butyric acid (IBA) than NAA. Growth of the regenerated shoots in a temporary immersion bioreactor resulted in remarkable increase in fresh, dry mass, leafy shoot ratio. The regenerated shoots from the temporary immersion bioreactor formed roots when transferred onto a medium with IBA. The regeneration protocol developed in the present study provides a basis for germ plasm conservation and elucidation of phytochemicals for further therapeutic investigation of this liverwort species. 

Index Terms—bioreactor culture, germplasm conservation, marchantia linearis, ms medium, growth hormones, in vitro culture

Cite: Remya Krishnan and K Murugan, "Establishment of In Vitro and Temporarily Immersed Cultures Using Rita of Marchantia Linearis Lehm. and Lindenb. A Liverwort," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 4, pp. 59-68, October 2014.
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