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Evolution of Mosquito Larvicidal Activity of Neem Seed Kernel Alkaloid against Anopheles stephensi

Neetu Arya
Pest Control and Ayurvedic Drug Research Laboratory, S.S.L. Jain P.G. College, Vidisha (M.P.) India
Abstract—Azadirachta indica (Neem) is commonly used in Indian traditional medicine for the treatments of variety of diseases. Over phytochemical studies of the Neem seed kernel showed the presence of alkaloid. Percentage of yield for methanol extract was 13.0%. The results of spectrum showed presence of sesquiterpene lactone group. The mosquito larvicidal activity of bioactive alkaloid isolated from Neem seed kernel have been tested against Anopheles stephensi. Three trials were performed for each concentration along with control and untreated. 24 h LC50 and LC90 values were determined using probit analysis method. The compound gave LC50 value to be 156.19 ppm for second instar larvae and 122.74 ppm for fourth instar larvae. It was noticed that fourth instar larvae are more susceptible than second instar larvae. The results obtained suggest that the bioactive compound of Azadirachta indica could be useful in the search for new larvicidal compound of plant origin. 

Index Terms—larvicidal, phytochemical, alkaloid, bioactive, anopheles stephensi, azadirachta indica

Cite: Neetu Arya, "Evolution of Mosquito Larvicidal Activity of Neem Seed Kernel Alkaloid against Anopheles stephensi," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 4, pp. 129-135, October 2014.
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