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An Efficient and Improved Method of In Planta Transformation in Arachis hypogaea. L

T G Umesh1 and Nagashree N Rao2
1. Department of Botany, Bangalore University, Bangalore-560056
2. Department of Biotechnolgy, RV College of Engineering, Bangalore
Abstract—Arachis hypogaea (Peanut) has long been the focus of conventional breeding efforts because of its importance as a source of high quality oil and proteins. Genetically modifying peanut plants by molecular techniques has been an important tool for introduction of agronomical useful genes responsible for improvement of economically important traits. In the present study, an improvised in planta Agrobacterium tumefaciens mediated genetic transformation was carried out in Arachis hypogaea L.cv. TMV-2. Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pCAMBIA 1301 was used for transformation of embryo axes with one of the cotyledon intact. The putative transformed embryos were germinated on MS basal medium supplemented with 40 mg/L hygromycin along with 250 mg/L cefataxime thus allowing direct selection of plants. After germination on MS medium, the putative transgenic plants were moved to sterile soilrite, maintained in greenhouse. The transgenic plants were validated by GUS histochemical assay and integration of transgene into peanut genome was confirmed by genomic DNA PCR. The optimized protocol could be applicable for other plant species for high transformation efficiency. 

Index Terms—in planta transformation, GUS, arachis hypogaea, hygromycin phosphotransferase

Cite: T G Umesh and Nagashree N Rao, "An Efficient and Improved Method of In Planta Transformation in Arachis hypogaea. L," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 4, pp. 166-173, October 2014.
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