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Proximate Composition, Characterization and Spectroscopic Analysis of Luffa Aegyptiaca Seed

Oli C C1, Onuegbu T U1, and Ezeudu E C2
1. Department of Pure and Industrial Chemistry, Nnamdi Azikiwe University, Awka, Nigeria
2. Department of Chemistry, Paul University, Awka, Nigeria
Abstract—This research aimed at determining the proximate composition of luffa aegyptiaca seed, characterization of its oil, and determination of the composition of the seed oil and seed coat oil using spectroscopic methods. The pulverized seed and oil from seed were analyzed. Results of the proximate analysis showed that the seed contains 7.5% moisture, 41% oil content. 2.0% ash, 7.5% crude fibre, 25.38% crude protein, 24.12% carbohydrate, and 567 kcal/g calorific value. Characterization of the seed oil showed that it contained 38.64 mg/g acid value, 19.32 mg/g free fatty acid, 1.33 meq/kg peroxide value, 23°C melting point and saponification value of 231.41. The oil from the seed as well as from the seed coat was subjected to spectroscopic analyses which include Fourier Transform Infra Red spectra (FTIR) and Ultra-Violent /Visible spectra (UV) analyses. The results obtained from the (FTIR) analysis showed the presence of the functional groups such as O - H, N - H, C - H, C = C, C ≡ C and their various absorption at different wave lengths. Ultra violent/visible spectra analysis showed that both the seed oil and seed coat oil contained mainly unsaturated compounds. 

Index Terms—luffa aegyptiaca, seed-oil, extraction, characterization, proximate composition, spectroscopic analysis

Cite: Oli C C, Onuegbu T U, and Ezeudu E C, "Proximate Composition, Characterization and Spectroscopic Analysis of Luffa Aegyptiaca Seed," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 3, No. 4, pp. 194-200, October 2014.
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