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Simultaneous Nitrification and Denitrification of Ammonical Wastewaters Using Bacillus Species SB1 Isolated from Domestic Sewage

Sheela. B1, Khasim Beebi. S2, and Yellaji Rao. O3
1 Department of Biotechnology, Gitam Institute of Science, Gitam University, Visakhapatnam
2 Department of Biotechnology, Gitam institute of Technology, Gitam University, Visakhapatnam
3 Safety and Environment, Synergies Castings Limited, Visakhapatnam
Abstract—Industrialization in the world increased the pollution levels in the environment making life more complicated. General awareness about pollution is increasing today. To control pollution levels, biological methods are eco-safe and economical. In this study a wild strain of Bacillus species was isolated and optimized for bioremediation of ammonia in flask cultures. The study was attempted to develop a process for ammonia bioremediation in a single stage aerobic nitrification and denitrification of ammonia. The results reveal that the optimum pH for the isolated Bacillus species strain was 8 and optimum temperature was 300C. At optimum pH and temperature this organism was able to remove 72.7% of ammonia in flask cultures supplemented with 860 mg l-1 of ammonia. The study on ammonia toxicity revealed that toxicity was due to increase in hydrogen ion concentration and osmosis.

Index Terms—ammonia bioremediation, Bacillus species, aerobic nitrification, denitrification, optimization, tolerance limit.

Cite: Sheela. B, Khasim Beebi. S, and Yellaji Rao.O, "Simultaneous Nitrification and Denitrification of Ammonical Wastewaters Using Bacillus Species SB1 Isolated from Domestic Sewage," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 1, pp. 17-21, January 2015.
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