1. How to submit my research paper? What’s the process of publication of my paper?
The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijlbpr@ejournal.net.
You’ll be given a paper number if your submission is successful. Your paper then will undergo peer review process, which may take approximately one and a half months under normal circumstances, three tops.
After blind peer review, you will receive the notification letter with the review result of your paper...
2. Can I submit an abstract?
The journal publishes full research papers. So only full paper submission should be [Read More]

Anticancer Activity of Methanolic Extract of Berberis aristata in MCF-7 Human Breast Cancer Cell Lines

Mamatha Serasanambati1, 2, Shanmuga Reddy Chilakapati1, 2, Pavan Kumar Manikonda1, and Jadeeswara Reddy Kanala1
1 SugenLife Sciences Pvt Ltd, Tirupati, Andhra Pradesh, India, 517505
2 Sri Venkateswara University, Department of Biochemistry, Tirupati, Andhra Pradesh, India, 517502
Abstract—Breast cancer is second most common in women and accounts for 23% of all occurring cancers in women. At present, more than 60% of the chemotherapeutic drugs are developed from plants and their derivatives, which can be used for development of anticancer drugs. An Indian barberry, Berberis aristata has been traditionally used for the treatment of inflammation, skin diseases, ulcers and cancers. In present study, the methanolic extract of stems of B. aristata, was used to investigate its anticancer activity in human breast cancer cell line (MCF-7). Different concentrations of the methanolic extracts (125, 250 and 500 μg/ml) were subjected to determine the cytotoxic effect by measuring the cell proliferation activity in MCF-7 breast cancer cell lines up to 48 h of incubation. The IC50 value for methanolic extracts identified was 220μg. Further, significant decreased (80%: p≤0.001) colony formation at 500μg/ml of methanolic extracts was noticed by soft agar assay in MCF-7 cells. However, in vitro scratch assay revealed the significant (p≤0.001) inhibition of cell migration up to 50% at 250μg of extracts. In addition, significant (68%) increase of apoptosis at 500μg of extracts in MCF-7 cells was evidenced by live/dead assay.

Index Terms—Berberis aristata, Cytotoxicity, Cell migration, Apoptosis, Anticancer activity

Cite: Mamatha Serasanambati, Shanmuga Reddy Chilakapati, Pavan Kumar Manikonda, and Jadeeswara Reddy Kanala, "Anticancer Activity of Methanolic Extract of Berberis aristata in MCF-7 Human Breast Cancer Cell Lines," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 1, pp. 31-35, January 2015.
Copyright © 2012-2015 International Journal of Life Sciences Biotechnology and Pharma Research, All Rights Reserved