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Establishment of Method for Protoplast Fusion with PEG-mediated between Jatropha curcas L. and Ricinus communis L.

Nootjaree Tudses1, Siripong Premjet2, 3, and Duangporn Premjet 1, 3
1 Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand
2 Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand
3 Center for Agricultural Biotechnology, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand
Abstract—Jatropha curcas L. and Ricinus communis L. belong to Euphorbiaceae and considered as important alternative biofuel. Somatic hybridization between J. curcas L. and R. communis L. was proposed to solve low genetic variability of J. curcas L. The intergeneric hybrid will provide a novel variety which perform as an annual crop and bear fruits to ease harvesting for commercial production. Protoplast fusion between J. curcas L. and R. communis L. to create intergeneric hybrid was attempted via PEG-mediated method. Mesophyll protoplasts of J. curcas L. and calli protoplasts of R. communis L. were used in this study. Concentration and molecular weight of PEG, fusion period, microfusion and macrofusion method were optimized. The highest (66%) viability of heterokaryon was obtained from using 30% PEG (MW6000) assisted with macrofusion method for 10 minutes.

Index Terms—Jatropha curcas L., Ricinus communis L. protoplast fusion, PEG-mediated method

Cite: Nootjaree Tudses, Siripong Premjet, and Duangporn Premjet, "Establishment of Method for Protoplast Fusion with PEG-mediated between Jatropha curcas L. and Ricinus communis L.," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 1, pp. 50-56, January 2015.
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