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Characterization of Amylase from Industrially Important Thermophilic Microorganism: Geobacillus thermoleovorans Strain Rekadwadsis

Bhagwan N. Rekadwad
School of Life Sciences, Swami Ramanand Teerth Marathwda University, Nanded, India
Abstract—The production of extracellular thermostable amylase by Geobacillus was detected on nutrient agar plates containing 2.0% soluble starch at 65 oC. Bacterium was produced 8, 578 U/mL amylase under SmF. Thermostable amylase (size 42 kDa) showed optimum temperature 68 oC at pH optima 7.5. Geobacillus amylase was stable at 90 oC temperature and retained its 85% initial activity. The thermostable amylase was retained stability and activity in the presence of various denaturing agents such as SDS, Triton X-100, Tween-60, Tween-80. Ca+2, Cu+2 and Co+2 ions increased activity, while Na2EDTA, Hg+2, Zn+2, Sn+2 showed inhibitory effects. The thermostable amylase was stable, compatible and works at 10 mg/mL concentration of laundry detergents (Ariel, Ghari, Surf Excel, Wheel, Tide, Nirma, Rin and Henko). The Km and Vmax values were 2.702 mg/mL and 7692.3 mmol respectively.

Index Terms—extremophiles, Geobacillus, amylase, ionic concentration, biocatalysts, thermostability

Cite: Bhagwan N. Rekadwad, "Characterization of Amylase from Industrially Important Thermophilic Microorganism: Geobacillus thermoleovorans Strain Rekadwadsis," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 1, pp. 26-30, January 2015.
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