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Media Optimization, Extraction and Partial Characterization of an Orange Pigment from Salinicoccus sp. MKJ 997975

Manish R. Bhat and Thankamani Marar
School of Biotechnology & Bioinformatics, D. Y. Patil University, Navi Mumbai, CBD Belapur 400614, Maharashtra, India
Abstract—In the current study an orange pigment was extracted from Salinicoccus sp. M KJ997975. Out of different solvents used acetone: methanol (5:5) (v/v) gave maximum extraction of the pigment. The extracted pigment showed λmax of 450 nm. Extract showed presence of caretenoids qualitatively. Sterile nutrient medium was found to be good choice of media for production of orange pigment. Various factors affecting pigment productivity were tested. Salinicoccus sp. M KJ997975 showed maximum pigment productivity at a pH of 7, temperature of 300C, with 2% inoculum size in 100 ml of media under shaking conditions of 120 rpm in presence of light. TLC analysis of extracted pigment showed Rf value of 0.65 in hypophase which suggested the presence of xanthophylls.

Index Terms—Microbial pigments, Salinicoccus sp., Carotenoid, Orange pigment, xanthophylls, pigment productivity

Cite: Manish R. Bhat and Thankamani Marar, "Media Optimization, Extraction and Partial Characterization of an Orange Pigment from Salinicoccus sp. MKJ 997975," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 2, pp. 85-89, April 2015.
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