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Biodiversity of Six Varieties of Mangifera indica Using RAPD

Hanumanth Kumar Gurijala1, Dileep Reddy Rampa2, and Pramoda Kumari Jasti3
1 Department of Biotechnology, Sri Venkateswara University, Tirupati, AP, India
2 Stem Cell Culture Research Laboratory, Stanley Medical College, Chennai, TN, India
3 Department of Microbiology, Sri Venkateswara University, Tirupati, AP, India
Abstract—The objective of our study was to identify the genetical diversity of six populations of Mangifera indica using Random Amplified Polymorphic DNA (RAPD) markers. Genomic DNA was extracted, subjected to PCR with the primers OPAB01, OPAB 04, OPAB 05, OPAB 06, OPAB 08, OPAB 09, OPAB 12, OPAB 14, OPAB 15, OPAB 19, OPAB 20, A2, A3, A 10, A 18, P10 and W18.Out of 17 RAPD primer tested, 15 showed polymorphism in the six populations of Mangifera. The means of each population contained in average 16.58 was found. From 15 primers 277 bands were found, out of them 94 bands shown polymorphism at the rate of 31.23%. Phylogenetic variability among all plants samples evaluated that Thothapuri was the most prominent resembling species with all of the remaining species following Malgoba, Rasapuri, Neelam, Badhami and Sendura.

Index Terms—mangifera, RAPD, genetical diversity, polymorphism

Cite: Hanumanth Kumar Gurijala, Dileep Reddy Rampa, and Pramoda Kumari Jasti, "Biodiversity of Six Varieties of Mangifera indica Using RAPD," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 2, pp. 100-103, April 2015.
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