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The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijlbpr@ejournal.net.
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Antibacterial Activity Pseudomonas sp. Isolated Rhizosphere against Methicillin Resistance Staphylococcus aereus from Clinical Samples

Asli kousha H. and Vatankhah M. R.
Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
Abstract—These methicillin-resistant Staphylococcus aureus (MRSA) is a substantial public health problem worldwide, causing significant morbidity and mortality. Methicillinresistant S. aureus is the leading cause of skin and soft tissue infection in patients reporting to emergency departments for treatment. The different types of biosurfactants with antimicrobial activity include lipopeptides, glycolipids, phospholipids and lipopolysaccharides. A few lipopeptides have been reported to produce by Gram-negative bacteria like Pseudomonas. The aim of this study was to antibacterial activity of a compound from Pseudomonas sp. against MRSA strains from clinical samples. The Pseudomonas sp. was isolated from Rhizosphere in North Iran. The antimicrobial activity of cell-free supernatant and partially purified bacteriocin was determined by well diffusion method. A total of 100 S. aureus isolated from clinical specimens. The bacteria were primarily identified by colony morphology, microscopy of Gram’s stain and routine biochemical tests and antibiotic disk sensitivity tests (Cefoxitin; Ceftriaxon; Amoxicillin; Cefotaxime; Co-trimoxazole; Penicillin; Tetracyclin; Azitromycin). Results of this study showed the isolate Pseudomonas sp. broadest antimicrobial spectrum against MRSA isolated clinical samples. However, the spectrum of inhibitory activity of these bacteria suggests a potentially useful means for controlling the growth of food-borne pathogens bacteria such: S. aereus and MRSA isolated food samples.

Index Terms—MRSA, rhizosphere, antiboitic activity, clinical sample.

Cite: Asli kousha H. and Vatankhah M. R., "Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 2, pp. 137-140, April 2015.
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