1.Department of Marine Living Resources, Andhra University, Visakhapatnam, 530003, India
2.Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530003, India
(PIC-4) (Genbank accession No: KF803248) was isolated from Coastal waters of Visakhapatnam. Cell free culture broth of the isolate exhibited antagonistic activity against pathogenic bacteria Aeromonas hydrophila
and E. coli
in agar well diffusion method with the diameter of inhibition zones 30mm and 36 mm respectively. Optimum temperature and time for growth and antibiotic production were standardised as 28°C and 48 hrs respectively and optimum NaCl concentration was determined as 1%, in addition to the LB broth medium. Two solvent fractions, F1 and F3 obtained on 100% hexane and ethyl acetate: hexane (1:1) respectively exhibited antibacterial activity against tested pathogens. F1, a colourless with low melting crystals found to have more antibacterial activity than that of F3, colourless short needles. Minimum inhibition concentration of both F1 and F3 were recorded as 32μg/ml on E. coli and 64μg/ml Aeromonas hydrophila
, Aeromonas hydrophila, E. coli, anttibacterial activity
Cite:M. Veerendra Kumar, Janakiram Pasupuleti, and Murali Krishna Kumar M., "Antagonistic Activity Exhibited by Crude Extracts of Pseudomonas aeruginosa (PIC-4) against Aeromonas hydrophila and E. coli," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 3, pp. 163-167, July 2015.