Screening and Characterization of Bacterial Biosurfactant from Bangkok and Vicinities
Manita Siriwong Na Ayudhya1,
Pumnat Chuenchomrat1, and
1.Department of Food Science and Technology, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand
2.Faculty of Science and Technology, Prince of Songkla University, Surat Thani, Thailand
—In this study, biosurfactant producing bacteria were screened from oil-contaminated areas in Bangkok and vicinities, Thailand. Isolated bacteria of 1,094 isolates were collected from the samples. Primary screening of biosurfactant producing bacteria was performed by oil agar plate method. From primary screening, 82 isolates showed dispersion zone surrounding colonies. Ten isolates with the highest RZC values were selected to study in the secondary screening. These isolates were separately cultured in NB. Tensiometer was used to measure surface tension of each cell free cultured broth. The decreasing of surface tension was observed in 4 isolates. BKG 04-005 isolate showed the lowest surface tension value of 18.58mN/m. According to the results, biosurfactant of BKG 04-005 was growth-associated product. To obtain biosurfactant, its cell free cultured broth was acidified and extracted by acid and a mixture of chloroform: methanol, respectively. The chemical structure of crude biosurfactant was identified as a lipopeptide by FTIR spectroscopy.
—screening, bacterial biosurfactant, surface tension
Cite:Manita Siriwong Na Ayudhya, Pumnat Chuenchomrat, and Pornpong Sutthirak, "Screening and Characterization of Bacterial Biosurfactant from Bangkok and Vicinities," International Journal of Life Sciences Biotechnology and Pharma Research, Vol. 4, No. 3, pp. 168-171, July 2015.