Introduction: Vancomycin resistant Enterococci (VRE) is a well-known cause of nosocomial infections worldwide. The major reservoir of VRE is patients with gastrointestinal colonization. Material and methods: Over a period of one year 200 stool samples from high risk patients were screened for VRE. Vancomycin drug resistance was confirmed by macrobroth dilution method(MIC), polymerase chain reaction(PCR). Typing of VRE strains was done by antibiotyping and DNA sequencing for epidemiological study. Results: Out of 200 stool samples. 12 samples (6%) had growth of VRE. All the isolates were found to be E.faecium and were multidrug resistant. MIC and PCR demonstrated Van A type of drug resistance in all the VRE strains. Antibiotyping showed four different antibiotype patterns labelled as PI-IV. Pattern PII(50%) was most common with most resistant strains. Genotyping by DNA sequencing showed 3 different genotype clusters labelled as G1-3, out of which G1 was the largest cluster with 9 VRE strains. Clustering of cases of VRE colonization with same antibiotye and genotype in ward 7 indicating transmission of same clone of VRE in the ward.Conclusion: Antibiotyping can be used as basic tool for epidemiological study. Combination of phenotypic and genotypic methods helps in better understanding of epidemiology of nosocomial pathogens in health care set up.