Background: Hysterectomy is one of the most common gynecologic operations performed in developed countries and is usually performed for benign disorders such as fibroids, endometrial hyperplasia, adenomyosis, endometriosis, uterine prolapse, dysfunctional uterine bleeding and cervical intraepithelial neoplasia. Hence; the present study was conducted for assessing the effect of Ultrasound Guided Rectus Sheath Block Versus Transverse Abdominis Plane Block for Pain Relief After Total Abdominal Hysterectomy. Materials & methods: The randomization was done using closed envelope method. The groups were: Group T (n=35) - to receive bilateral TAP block and Group R (n=35) - to receive bilateral Rectus Sheath Block. This study was conducted on 70 women of ASA physical status I or II who were posted for elective TAH procedure. Patients were randomly allocated to the two study groups: one with 35 patients received USG guided bilateral TAP block (Group T) while other group with 35 patients received USG guided bilateral RS block post-operatively (Group R). Patients selected for surgery were admitted 24 hours prior to surgery. Age, gender, weight, ASA physical status were noted down in all patients. A thorough history Sample were sent for all required haematological and biochemical investigations. On the day of surgery, after confirming NPO status, Premedication was done with injection Ondansetron 0.10 to 0.15 mg/ kgIV, injection Pantoprazole 0.6 to 1.2 mg/kg IV and injection Ceftriaxone 30-40 mg/kg IV after performing sensitivity test. Local Anaesthetic Sensitivity Testing was also be done with a test dose of 2% plain Lignocaine HCl. Results: Mean VAS scores ranged from 0.23±0.43 (24hr) to 2.60±1.19 (4 hr) in Group T and from 0.37±0.65 (24 hr) to 3.66±1.71 (4 hr) in Group R. At all the follow-up intervals mean VAS scores were lower in Group T as compared to that in Group R and this difference was significant statistically too at 4, 8 and 16 hr follow-up intervals. Rescue analgesia was required in 97.1% of Group T and all the patients (100%) of Group R, statistically, there was no significant difference with respect to number of patients requiring rescue analgesia (p=0.314). Mean duration of analgesia was 8.17±4.74 hours in Group T as compared to 3.89±1.82 hours in Group R. Statistically, this difference was highly significant. Conclusion: The present study was carried out to compare the post-operative analgesia between USG guided Transverse Abdominis Block and Rectus Sheath Block in Total Abdominal Hysterectomy. The findings of the study showed that both TAP as well as RS blocks were safe and effective for post-operative pain in patients undergoing hysterectomy yet TAP block had an edge over RS block in view of longer duration of analgesia and lower requirement of rescue analgesic dosages.