This prospective observational study explores the role of Diffusion Weighted Imaging (DWI) in characterizing breast masses and differentiating benign from malignant lesions. The study included 54 patients with 59 breast lesions, who met the inclusion criteria based on X-ray mammography and/or sonomammography. The aim was to assess the utility of DWI, measured via the apparent diffusion coefficient (ADC) values, in distinguishing between benign and malignant lesions. The results indicate that malignant lesions exhibit significantly lower ADC values than benign lesions. An optimal ADC threshold of 0.998 × 10^-3 mm²/s for minimum ADC values and 1.209 × 10^-3 mm²/s for mean ADC values were identified, demonstrating high sensitivity (92% and 96%, respectively) and specificity (94% and 91%, respectively). The findings suggest that DWI, particularly ADC value analysis, can be a reliable diagnostic tool in differentiating benign from malignant breast lesions, offering high diagnostic accuracy with minimal reliance on contrast media, and is especially beneficial for patients with contraindications to contrast agents.