Background: Fungi are eukaryotic organisms which possess a rigid cell wall containing chitin, mannose, and other polysaccharides with cytoplasmic membranes containing sterols. They possess true nuclei with a nucleolar membrane and paired chromosomes and divide asexually, sexually, or by both processes. Objective: to study Fungal Disease Profile in a tertiary care hospital New Delhi. Methods: The present study was a prospective comparative study conducted in the Department of Microbiology, Lady Hardinge Medical College, New Delhi from November 2022 to March 2024. A total of 100 patients, clinically and microbiology diagnosed with fungal infections were included in this study. Result: Maximum number of patients belonged to 20-49 years (44%) age group, with slight male preponderance (male: female:1.04:1).The most common presenting complaint in the study population was fever present in 27(27%) followed by discoloration in 22%. There was no underlying disease in 81 % patients. Most of the patients had complaints related to respiratory system (11 %) followed by Taenia infection (3%). There were no apparent risk factors in 28 % patients, Catheterisation (15%) was the major risk factor followed by presence of comorbidities, intubation, low birth weight, increased age, malnutrition, Neutropenia, Post-operative history, prior antifungal, History of blood transfusion, immunosuppressive therapy (chemotherapy), Antitubercular drug intake, oral ulcers and hyperalimentation and positive HIV status. Most patients had involvement of skin, appendages and soft tissues (36%) followed by respiratory system (26%), genitoirunary system (7%), gastrointestinal system (5%),CNS and ENT and PNS system (4% each ). Nail clippings comprised 27% of the samples followed by blood (17%), urine (20 %) respiratory samples (19%) which included sputum (11%), Tracheal aspirate (4%) and BAL (3%) followed by skin scrapings (7%), while hair clipping, peritoneal drain fluid, and pus were 1% each. Conclusion: The findings advocate for heightened clinical suspicion, improved diagnostic capabilities, and judicious use of antifungal therapies to address the challenges posed by these infections.