Background: Physiological weight loss in newborns is a common occurrence in the first few days after birth, particularly among exclusively breastfed infants. Understanding the factors influencing this weight loss is crucial for ensuring effective breastfeeding practices and neonatal health. Aim & Objective:Objective of this study was to assess the pattern of weight loss in exclusively breastfed term babies in initial 72 hours of life and to study association of weight loss in exclusively breastfed term babies with selected clinical variables namely – Parity, Initial birth weight of neonate, Adequacy of breast feeding. Methods:The Department of Pediatrics at RMRI carried out a prospective observational study between May 2023 and November 2024. A total of 170 healthy, term newborns who were fed only breast milk were included in the study. Information on neonatal parameters (birth weight, sex), breastfeeding adequacy, and maternal variables were gathered. For the first 72 hours, weight loss was assessed every 12 hours. Results:The study found that 78.8% of neonates experienced weight loss between 5-10% of their birth weight, with a mean percentage loss of 7.89% (SD ±2.18). Inadequate breastfeeding was significantly associated with greater weight loss (p=0.001), while primigravida mothers had infants with higher weight loss compared to multigravida mothers (p=0.004). Conclusion:The findings highlight the critical role of breastfeeding adequacy and maternal parity in influencing neonatal weight loss patterns. Early identification and intervention for at-risk infants are essential to optimize breastfeeding outcomes and improve neonatal health.