Background: Laparoscopic cholecystectomy is the gold standard for gallbladder removal, with recent advances introducing Single Incision Laparoscopic Cholecystectomy (SILC) as an alternative to the Standard/four-Port Laparoscopic Cholecystectomy (SLC). This study aims to compare the outcomes of SILC and SLC. Methods: A total of 100 patients were enrolled and equally divided into two groups: Group A (SLC) and Group B (SILC). Demographic data, operative time, postoperative pain, complications, hospital stay, and cosmetic outcomes were compared between groups. Results: Most patients in both groups were aged between 31–50 years (SILC: 56%, SLC: 54%), with a female predominance (SILC: 70%, SLC: 76%). The mean time to gallbladder removal was significantly longer in the SILC group (24.38±3.53 minutes) compared to the SLC group (16.4±3.28 minutes) (p<0.001). Port site closure was faster in SILC (3.18±0.77 minutes) than in SLC (3.84±0.79 minutes) (p<0.001). Pain scores were significantly lower in SILC on both the first and second postoperative days (p<0.001). No vascular injuries were reported. Ductal injuries occurred in 6% of SLC patients but none in SILC. Biliary leakage (4%) and biliary peritonitis (4%) were similar in both groups. Seroma formation was slightly higher in SILC (6%) compared to SLC (2%). Hospital stays were comparable between groups (p=0.838). Cosmetic outcomes were significantly better in the SILC group (7.48±0.86 vs. 4.98±0.80, p<0.001). Conclusion: SILC is a safe and effective alternative to standard laparoscopic cholecystectomy, offering the advantages of less postoperative pain and superior cosmetic outcomes, though with a slightly longer operative time. With appropriate patient selection and surgical expertise, SILC can be considered a favorable option for gallbladder surgery.