Background: Laboratory testing has become an integral and essential part of health care system, which implies a need of quality assurance in this field. Quality indicators (QI) serve as a matrix for evaluation of quality in laboratory services. Present study has focused on assessment and analysis of QI applicable in extra analytical phase (pre and post analytical) of laboratory sample testing.Material Method: The present study conducted for the period of one year (Jan 24 to Dec 24) considering testing parameters which are under NABL scope. Total five QI in pre analytic phase and three QI in post analytic phase were evaluated. Percentage of each QI calculated according to formula set by quality policy of the laboratory. Results: Out of total 67326 samples received for hematological analysis, sample rejection rate was 2.32%, with clotted samples were the most common cause. Frequency of incomplete TRF was 13.01%.Among post analytical QI, frequency of TAT outlier test report was 0.6%; frequency of critical value reporting was 3.98%; frequency of revised reports was 0%. Sigma score for individual QI was calculated which was ranging from 2.7 to 4.1.Conclusion: Extra analytical phase (Pre and post) has impact on overall performance of laboratory services and is as important as analytical phase of sample testing. In present study errors in pre analytical phase were more frequent (15.33%) as compared to post analytical phase (4.58%). Analysis of QIs provides an objective assessment of quality of laboratory testing and provides insight about area of improvement.